High Homology-Directed Repair Using Mitosis Phase and Nucleus Localizing Signal
نویسندگان
چکیده
منابع مشابه
Noncanonical views of homology-directed DNA repair.
DNA repair is essential to maintain genomic integrity and initiate genetic diversity. While gene conversion and classical nonhomologous end-joining are the most physiologically predominant forms of DNA repair mechanisms, emerging lines of evidence suggest the usage of several noncanonical homology-directed repair (HDR) pathways in both prokaryotes and eukaryotes in different contexts. Here we r...
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We develop a method for measuring and localizing homology classes. This involves two problems. First, we define relevant notions of size for both a homology class and a homology group basis, using ideas from relative homology. Second, we propose an algorithm to compute the optimal homology basis, using techniques from persistent homology and finite field algebra. Classes of the computed optimal...
متن کاملDNA Damage, Homology-Directed Repair, and DNA Methylation
To explore the link between DNA damage and gene silencing, we induced a DNA double-strand break in the genome of Hela or mouse embryonic stem (ES) cells using I-SceI restriction endonuclease. The I-SceI site lies within one copy of two inactivated tandem repeated green fluorescent protein (GFP) genes (DR-GFP). A total of 2%-4% of the cells generated a functional GFP by homology-directed repair ...
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The promyelocytic leukemia protein (PML) is a tumor suppressor critical for formation of nuclear bodies (NBs) performing important functions in transcription, apoptosis, DNA repair and antiviral responses. Earlier studies demonstrated that simian virus 40 (SV40) initiates replication near PML NBs. Here we show that PML knockdown inhibits viral replication in vivo, thus indicating a positive rol...
متن کاملCorrection: DNA Damage, Homology-Directed Repair, and DNA Methylation
[This corrects the article DOI: 10.1371/journal.pgen.0030110.].
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2020
ISSN: 1422-0067
DOI: 10.3390/ijms21113747